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Home > Publications > Comprehensive Molecular Characterization of Gastric Adenocarcinoma, 2014

Comprehensive Molecular Characterization of Gastric Adenocarcinoma

Nature (2014) Received 21 February 2014 | Accepted 13 May 2014 | Published online 23 July 2014

[doi:10.1038/nature13480]

Abstract

Gastric cancer is a leading cause of cancer deaths, but analysis of its molecular and clinical characteristics has been complicated by histological and aetiological heterogeneity. Here we describe a comprehensive molecular evaluation of 295 primary gastric adenocarcinomas as part of The Cancer Genome Atlas (TCGA) project. We propose a molecular classification dividing gastric cancer into four subtypes: (1) tumours positive for Epstein–Barr virus, which display recurrent PIK3CA mutations, extreme DNA hypermethylation, and amplification of JAK2, CD274 (also known as PD-L1) and PDCD1LG2 (also known as PD-L2); (2) microsatellite unstable tumours, which show elevated mutation rates, including mutations of genes encoding targetable oncogenic signalling proteins; (3) genomically stable tumours, which are enriched for the diffuse histological variant and mutations of RHOA or fusions involving RHO-family GTPase-activating proteins; and (4) tumours with chromosomal instability, which show marked aneuploidy and focal amplification of receptor tyrosine kinases. Identification of these subtypes provides a roadmap for patient stratification and trials of targeted therapies.

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These data represent a data freeze from February 2, 2014. Please note that more recent data are available via the TCGA Data Portal.

The data are supported by different organizations. All data marked by [DCC] are DCC-validated archives. All data marked by [Supplementary] were created by the manuscript authors and you should contact the corresponding author for support.

Participant List, Sample List, BAM File List, and Full Listing of TCGA Archives for Data Freeze

Clinical

Microsatellite Instability

Mutations

IlluminaGA RNASeq and IlluminaHiSeqRNASeq VCF files

Reverse Phase Protein Array (RPPA) Expression

RNA Expression from IlluminaGA RNASeq and IlluminaHiseq RNASeq

SNP and Copy Number variation from Affymetrix SNP6 and IlluminaHiSeq Low Pass Whole Genome sequencing

miRNA from Illumina HiSeq 2000 and Illumina GA IIx

Methylation from Illumina Infinium Human Methylation450 and Methylation27

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